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How peptide reconstitution math works
Concentration equals vial strength (mg) divided by the water added (mL). On a U-100 insulin syringe, 100 units equals 1 mL, so each unit is 0.01 mL and the milligram amount per unit is the concentration divided by 100. To hit a target dose, divide the dose by the mg-per-unit value. Adding more bacteriostatic water lowers the concentration and raises the number of units per dose, which improves dosing precision for very small research amounts.
Frequently asked questions
How do I calculate peptide reconstitution?
Divide the vial strength in milligrams by the bacteriostatic water added in milliliters to get concentration in mg/mL. A 5 mg vial with 2 mL gives 2.5 mg/mL. On a U-100 syringe scale 100 units equal 1 mL, so the volume for any research reference amount is that amount divided by the concentration. This calculator does the arithmetic for laboratory preparation only.
What is bacteriostatic water?
Bacteriostatic water is sterile water with 0.9% benzyl alcohol that inhibits microbial growth, used to reconstitute lyophilized research peptides. The volume you add sets the final concentration. Concentration equals peptide mass divided by diluent volume: a 5 mg vial reconstituted with 2 mL gives 2.5 mg/mL, or 2,500 mcg/mL.
How many syringe units match a research reference amount?
It depends on both the vial strength and the water volume added. Enter the vial mg, the water mL, and the reference amount from your research protocol, and the calculator returns the matching volume in mL and in U-100 syringe units, where 100 units equal 1 mL. It is measurement arithmetic for laboratory work, not guidance for use in any person or animal.
Does adding more water change the amount of peptide?
No. Adding more water lowers the concentration but does not change the total milligrams of peptide in the vial. A more dilute solution means a larger volume corresponds to the same reference amount, which can make very small laboratory measurements more precise. Record the final concentration on the vial label so every later calculation uses the right figure.
Per-compound reconstitution reference · Full reconstitution guide · Research guides
External references: Bacteriostatic water (Wikipedia) · U.S. Food and Drug Administration